Introduction of DNA Fingerprinting,DNA Profiling,It’s Steps !!

DNA FINGERPRINTING DNA fingerprinting is a method of identification that compares fragments of DNA with the exception of identical siblings; the DNA of each person is unique. Process of DNA finger printing was invented by Alec Jeffreys in 1985. It is a method for identifying individual by their unique pattern of DNA banding STEPS OF […]

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DNA sequencing,Sanger sequencing,Illumina Sequencing !!

DNA SEQUENCING                         DNA sequencing is a process of determining the precise order of nucleotides within a DNA molecule. It includes any technology that is used to determine the order of the four bases. Adenine (A) Guanine (G) Cytosine(C) Thymine (T) Sequencing is a complex method. It requires breaking the DNA of the genome into many […]

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Polymerase Chain Reaction,PCR !!

PCR In 1980s, an important revolutionary technique of molecular biology PCR or Polymerase chain reaction was first described. Kary Mullis has been awarded for PCR in 1993. PCR takes place without living cells in the tube with the help of special heat stable DNA polymerase enzyme. Here the primer strand in the form of oligonucleotides […]

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Gel Electrophoresis,It’s type,SDS-PAGE Electrophoresis !!

GEL ELECTROPHORESIS Gel electrophoresis is a method that separates macromolecules, either nucleic acids or protein. TYPES OF GEL ELECTROPHORESIS There are some types of gel electrophoresis. Slab Gel Electrophoresis (SGE). Polyacrylamide Gel Electrophoresis (PAGE). Agarose Gel Electrophoresis (AGE). Pulsed Field Gel Electrophoresis (PFGE). SLAB GEL ELECTROPHORESIS Slab separations are carried out on a thin flat […]

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Introduction of Biotecnology,Recombinant DNA Technology,Restriction Enzyme !!

BIOTECHNOLOGY Biotechnology is the study and manipulation of any living thing or their component molecules, cells, tissues or organs. GENETIC ENGINEERING Deliberate modification of an organism’s genetic information by directly changing its nucleic acid genome is called genetic engineering. RECOMBINANT DNA TECHNOLOGY Recombinant DNA technology is a technology which allows DNA to be produced through […]

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