TYPES OF ENZYME
Transfer hydrogen and oxygen atoms or electrons from one substrate to another
Transfer phosphate or methyl group from one substrate to another
Hydrolysis a substrate
Change molecular form of the substrate
Non-hydrolytic removal of a group or addition of a group to a substrate
Join two molecules by the formation of new bonds
NATURE OF ENZYME ACTION
Each enzyme has an active site to which substrate binds and forms a short lived highly reactive enzyme substrate complex.
FACTOR AFFECTING ENZYME ACTIVITY
Enzymes generally function in a narrow range of temperature
Temperature at which the enzyme shows its highest activity known as optimum temperature.
Hydrogen ion concentration (pH)
Every enzyme has an optimum pH when it is most effective
A rise or fall in pH reduces enzyme activity
Concentration of substrate
Increase in substrate concentration, increases the velocity of the enzymatic reaction
Km (Michaels constant)
Concentration of substrate at which half the maximum velocity of the enzyme reaction is attained.
This is the general notes on enzyme types and the nature of enzyme action.
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